Chemical and physical properties of fractionated chromatin.

نویسندگان

  • E M Berkowitz
  • P Doty
چکیده

Sonicated chicken reticulocyte chromatin was fractionated into transcriptionally active and transcriptionally repressed components. The active fraction is 8% of the whole chromatin but contains 70% of the newly synthesized chromosomal RNA. This RNA has five times as many hemoglobin RNA sequences as does the RNA in the repressed fraction. The amount of the active fraction in the chromatins of several tissues correlates with their synthetic activity. The molecular weight of the DNA of the repressed fraction is approximately twice that of the active fraction. Moreover, the configuration of repressed chromatin is much more compact, consistent with a much larger sedimentation constant. The transcriptionally active fraction displays a 6 degrees lower melting profile and is highly susceptible to DNase I relative to the repressed fraction. The active fraction contains twice as much non-histone protein and 15% less histone than the repressed fraction and is lacking the lysine-rich and much of the arginine-rich histones.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

THE EFFECT OF ADRIAMYCIN ON SALT FRACTIONATED CHROMATIN

In this study calf thymus chromatin was fractionated into active (S1 and S2) and inactive (P 2) chromatin. Then the interaction of an anthracyc1ine antibiotic, adriamycin, with these fractions was investigated employing absorption and difference UV Nis spectroscopy and SDS and agarose gel electrophoreses. The results suggest that the binding of adriamycin to the S 1 fraction is slight but ...

متن کامل

Fractionation of Iranian Beef Tallow - Chemical and Physical Evaluations of the Fractions

ABSTRACT: Large quantities of beef and mutton tallow are available in Iran every year. If these products are processed and formulated properly and scientifically, the need to import oils and fats in a substantial quantities might be reduced. The aim of this study is to fractionate beef tallow into different fractions and carry out physical and chemical tests on each isolated fraction. Beef fat ...

متن کامل

Influence of organic compost compounds on soil chemical and physical properties

This study was conducted to evaluate the impact of municipal waste compost and manure on soil chemical and physical properties quality and crop production in Sari city (north of Iran). In this study, the effect of compost and manure (cow and sheep) on the quality of soil organic material with experimental measurements was investigated. An experiment was conducted in a completely randomized desi...

متن کامل

Wildlife and Livestock Grazing Effects on Some Physical and Chemical Soil Properties (Case Study: Kalmand-Bahadoran Arid Rangelands of Yazd Province)

Owing to the importance and necessity of identifying soil properties in arid rangeland management, and the variableeffects of different kinds of herbivores on soil, this study compares some of the physical and chemical properties ofsoil between two regions experiencing livestock and wildlife grazing in the desert rangelands of Kalmand-Bahadoranin Yazd province. The key areas in two regions expe...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Proceedings of the National Academy of Sciences of the United States of America

دوره 72 9  شماره 

صفحات  -

تاریخ انتشار 1975